Describe two differences between DNA and RNA.

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1. Describe two differences between DNA and RNA. Describe two differences between transcription and translation.
2. Where does DNA replication take place in a fungus? Where does DNA replication take place in a bacterium?
3. Template strand of DNA: ATAAAGTACGGGCCCAGCATATCGGCCATTCAGCCGTGC. a) Following transcription, what is the total number of codons in the mRNA transcript? b)What codon number is the start codon in this mRNA transcript? b) What codon number is the stop codon in this mRNA transcript? d) Following translation of this mRNA transcript, name the amino acids in order for the protein.
4. What type of horizontal gene transfer is mediated by a bacteriophage?
5. What type of mutation changes a normal codon into a stop codon and stops the synthesis of the protein?
6. Describe the function of restriction endonucleases.
7. What type of DNA is used in genetic engineering that has the advantage of being free of intervening sequences known as introns?
8. Describe the three steps in one cycle of the polymerase chain reaction (PCR).
9. What is the difference between ex vivo and in vivo gene therapy?
10. What type of analysis can determine hundreds or thousands of genes than can be transcribed in a given tissue on a single chip?

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