Discuss the merits of your restriction enzyme cloning protocol compared with other methods such as the Cre-LoxP system, and outline scenarios in which you might prefer one method over another.

You are investigating the function of a novel gene, called SBS1. You have a donor vector which hosts the sequence of SBS1 flanked by a pair of BglII sites. The gene contains BamHI and EcoRI sites internally in its open reading frame. You have an accepting vector with a multiple cloning site (MCS), which contains both BamHI and EcoRI sites. Devise a protocol to clone the SBS1 gene into the MCS of the accepting vector, ensuring that the entire length of the gene is included. Discuss the merits of your restriction enzyme cloning protocol compared with other methods such as the Cre-LoxP system, and outline scenarios in which you might prefer one method over another.

Complete as a word-processed document using 1.5 line spacing, Arial font (10 pt)
and margins of 2.5 cm on all sides of an A4 page.

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