Here is an outline of what my topic is supposed to be about. My paper should elucidate how this emerging technology
can protect public health from an environmental health perspective:
INTRO: Describe what LAMP is and how it is used to detect Salmonella in various food matrices.
METHODS: How does LAMP work? What steps are taken to run a LAMP sample to test it for Salmonella?
RESULTS: How do you interpret the results from a LAMP sample to test it for Salmonella?
DISCUSSION: Why is LAMP better or worse for detecting Salmonella from food?
*Sensitivity/Specificity of the assay.
*Limit of Detection, and compare these measures to PCR or other methods used to traditionally detect
Salmonella!
CONCLUSION: Summarize why LAMP is used and how this technology can protect public health.
This is what I have so far:
1. Salmonella- is a bacteria that causes infection to humans by eating contaminated foods with animal feces. Salmonella has been in meat products, poultry products, raw or undercooked eggs, and dough, fruits, and dairy products. The Rapid Microbiological Method that scientists have used is called Loop-mediated isothermal amplification (LAMP). This mechanism is an alternative to a polymerase chain reaction (PCR) for pathogen detection in clinical specimens and food matrices.
2. A major advantage of LAMP over PCR is the high tolerance to biological substances, such as whole blood and urine, commonly found in clinical specimens. Another advantage of LAMP is that it has shown a greater tolerance to potential assay inhibitors (e.g. soil, culture media, humic acid) than PCR. Salmonella LAMP assays have been used in a wide variety of food matrices, including all the major food categories linked to Salmonella outbreak-associated illnesses, for example, produce, eggs, chicken, pork, and beef. This method would make an impact on environmental health because this method is reliable, robust, and can help detect Salmonella in order to prevent foodborne illnesses.
References:
Fang J, Wu Y, Qu D, et al. Propidium monoazide real-time loop-mediated isothermal amplification for specific visualization of viable Salmonella in food. Lett Appl Microbiol 2018. [Epub ahead of print].
Hu L, Ma LM, Zheng S, et al. Development of a novel loop-mediated isothermal amplification (LAMP) assay for the detection of Salmonella ser. Enteritidis from egg products. Food Control 2018;88:190–197. Crossref, Google Scholar
CDC. Reports of Selected Salmonella Outbreak Investigations. 2018.
Here are additional links to use for the essay. This will help to discuss the sensitivity/specificity of the assay.
1. https://www.fws.gov/aah/PDF/QI-Terms%20and%20Defs.pdf
2. https://www.fws.gov/aah/PDF/SandS.pdf
3. https://pubmed.ncbi.nlm.nih.gov/32964461/
4.https://www.fws.gov/aah/PDF/QI-Terms%20and%20Defs.pdf
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