-: What are the overall reactions of EcoRV and DpnI, and why were they useful for the cloning strategies in this lab?
– Explain the features of DNA spectra and what parts of the DNA molecule are responsible for these features. Include a chemdraw figure demonstrating the Watson-Crick base pairs.
– Explain duplication and error rate numbers.
-Would the experiment have failed if the PNK (Polynucleotide kinase) reaction was omitted? Explain why or why not?
– Compare the transformation efficiencies result to the value of 0.6 – 1.0 x 109 cfu/µg pUC19 reported by New England Biolabs when transforming a 100% circularized pUC19 vector. Explain your results.
I also upload the lab manual of my lab and my lab report on files section: Please look at that before you do my assigment because it was alot of information. Thank you
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