Just write the Introduction and discussion section
Method and result section is provided in the uploding file
Use this website to find the below paper
Website: http://sci-hub.se/
Here are some of the paper that should include in the introduction and dicsussion section
The genes of the Green Revolution by Peter Hedden (2003)
Mendel’s Stem Length Gene (Le) Encodes a Gibberellin 3 Beta-Hydroxylase by Diane R. Lester, John J. ROSS, Peter J. Davies, and James B. Reid (1997)
Mendel’s Genes: Toward a Full Molecular Characterization by James B. Reid and John J. Ross (2011)
Gibberellin metabolism: new insights revealed by the genes Peter Hedden and Andrew L. Phillips (2000)
Gibberellin signaling in plants Jean-Michel Davière and Patrick Achard (2013)
Mendel’s dwarfing gene: cDNAs from the Le alleles and function of the expressed proteins DAVID N. MARTIN, WILLIAM M. PROEBSTING, AND PETER HEDDEN (1997)
Please follow the formate which is mention below for the introduction and discussion
Introduction:
The introduction should follow the structure of an inverted triangle. Start with the big-picture, theoretical background and then narrow the material down until you reach your experimental purpose, hypothesis, predictions, and approach.
First two to three paragraphs:
Background on the experiment – Focus on the important overall theoretical questions of the experiment and center on the background information that elucidates those questions. Set up the background in such a way that it naturally leads the reader to the hypothesis. If you do not support your hypothesis in your introduction, then your introduction needs massive reworking.
For this experiment, perhaps you would start with the importance of agriculture (feeding the world!) and move on to how dwarf plants provide certain agricultural benefits. Then you could transition the end of that paragraph to the source (genetic) of dwarfism in plants. The next paragraph could go into the genetic, enzymatic, and physiological pathways that lead to dwarfism, focusing on GA production/response and the role the Le gene plays in that pathway..
Last paragraph
Remember this is a two part experiment: GA response and Genotypic Analysis
Purpose/question of the experiment
Determine if mutations in the Le gene may be the cause of dwarfism in our dwarf pea varieties.
You will need to generate hypotheses/predictions to address the above purpose/question. For example:
As previous research has identified mutations in the Le gene that reduce GA1 production producing dwarf plants, we hypothesize that dwarf pea plant varieties that respond to exogenous GA application will have mutations in their Le coding sequences which alter the enzymatic ability of GA 3-oxidase (GA 3b-hydroxylase) resulting in reduced GA1 production.
Give the reader a preview of the experimental approach you will be using to address the above purpose/question and hypotheses. You should not give a great deal of detail, it is not the methods. It is just an overview of the approach you will be taking. For example:
To determine if the commercial pea line was a GA responsive dwarf or not, plant growth was measured with and without gibberellin treatment.
Discussion:
The discussion is the most important part of your paper. I expect you to show me that you can think/analyze like a scientist in this section. As this section is all about your scientific capability, this is the one part of the paper for which I will give limited aid.
The discussion should start out with a “major findings” paragraph that reminds the reader of the experimental question/hypothesis and then presents the major findings of the study. From this paragraph, the discussion should widen out to compare your findings with the work of other scientists, present experimental limitations, and explore future experiments/questions raised by your results. You should end the discussion with a broad, “big picture” paragraph which restates your major conclusions and elucidates the significance of your findings.
Discuss whether you supported your hypothesis or achieved your purpose.
Draw conclusions from your experiment, and use results to back up those conclusions.
Compare your results to other scientific literature.
Discuss possible limitations to your experimental design, especially if those limitations have affected your results or limited the conclusions you can draw. If there are errors that affect the conclusions or outcome of the results, then include them and discuss how the results reflect these errors and how they affected your conclusions. If errors do not affect the conclusions, you do not have to mention them. If you are stating that some result you see is due to error, you need to back that statement up with observations from your data. Don’t just throw out error as an explanation without a sound basis for where the error occurred and what effect it had on the final results. In other words, I don’t want you to list any possible error. I often see a huge list of errors (none of which actually occurred); the student just lists anything that could ever go wrong with the experiment. I do want you to acknowledge legitimate errors or limitations, but not hypothetical errors.
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