Identify one pitfall in Gram staining not discussed in lecture.

Discussion post #1
1. What are the steps to Gram staining? What is the purpose for each Gram staining reagent? Be as detailed as possible.
2. Identify one pitfall in Gram staining not discussed in lecture. What could be done to correct this pitfall? What can be done to prevent it from occurring in the future?
3. Why is Gram staining an essential tool in microbiology?
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Discussion post #2
Answer the following questions:
1. Describe the steps for completion of the indole test. Include information on inoculation, incubation, and result interpretation.
2. What is the purpose of using biochemical tests when identifying an unknown organism?
3. Pick any test (except indole), identify the test and its purpose, and describe the steps for inoculation, incubation, and result interpretation. Be sure to include all necessary reagents.
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Discussion post #3
1. Which topic from the course is your favorite (so far)? Why is it your favorite topic? How will you use this topic in your future career?
2. Which topic was the most difficult for you? What steps did you take to improve your understanding of the topic?
3. How has the COVID-19 pandemic increased the relevance of MCB 2010L?
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Discussion post #4
1. List and describe each stage of the bacterial growth curve. What are the key features of each stage?
2. Describe the difference between thermal death point and thermal death time. How can you determine the thermal death point of a given bacteria?
3. Describe the disk diffusion method. Why is it important? How can the disk diffusion method determine antibiotic susceptibility?
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Discussion post #5
1. Describe the differences between Gram positive and Gram negative bacteria. How can the differences be used to treat bacterial infections?
2. What is the difference between antiseptics and disinfectants? Name an antiseptic and disinfectant you use on a daily basis.
3. Describe the skills that you learned/utilized to complete your unknown project. What did the project teach you?
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