Your submission should include:
1-2 paragraphs describing how you conducted your experiment – i.e. what is often called methods and materials.
1-2 paragraphs describing and detailing the results of your experiment – i.e. what is sometimes called discussion and conclusions.
1 or 2 photos showing the results = extracted DNA – i.e. provide some visual “data” to support your discussion and conclusions.
Your report should be a minimum of 250 words and include at minimum 1 ‘real’ photo NOT taken from the web.
This assignment is due on 9/30 at midnight.
Download this excellent infographic from the NIH showing how to conduct this experiment in 10 easy steps using basic household items.
A Reminder from the Lab 4 Instructions:
Lab 4: Cell Biology and DNA
When you added the salt and detergent mixture to the smashed strawberries, the detergent helped lyse (break open) the strawberry cell membranes, releasing the DNA into solution, whereas the salt helped create an environment where the different DNA strands could clump, making it easier for you to see them.
When you added the salt and detergent mixture, you probably mostly just saw more bubbles form in the bag because of the detergent.
After you added the cold rubbing alcohol to the filtered strawberry liquid, the alcohol should have precipitated the DNA out of the liquid while the rest of the liquid remained in solution.
You should have seen the white/clear gooey DNA strands in the alcohol layer as well as between the two layers. A single strand of DNA is extremely small – too small to see with the naked eye – but because the DNA clumped in this activity you were able to see just how much of it three strawberries have when all of their octoploid cells are combined!
Remember “octoploid” means they have eight copies of their genome.
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