Protein localisation using green fluorescent protein chimeras

Words: 334
Pages: 2
Subject: Premium Writing

Hello,

I need to write a Lab Report on the ‘Protein localisation using green fluorescent protein chimeras’ pratical.

I’ve attached the guidelines and screenshots of information that was used throughout the practical. The generalt premise is that we were made to transfect cells with and generate a hypothesis as to where our GFP-Chimeras were localising.
– We hypothesised that they were localising with Tublin / Microtubulies
– Based on the research given to us we decided to test this hypothesis with the drug Nocodazle as it binds to the Tubulin Dimers and prevents mitosis by stopping them from entering metaphase; this induces apoptosis in tumour cells such as HeLa Cells. If TAT1 co-localises with GFP then the Nocodazle with show that rather than it just being a visual correlation.
– References: (Role of kinesin-1 and cytoplasmic dynein in endoplasmic reticulum movement in VERO cells /// https://europepmc.org/article/PMC/553680https://europepmc.org/article/PMC/553680)

I just need an Introduction, Methods and potentially a Resutls and Discussion section if possible. A Results section would be nice if you could do it but I understand if not.
– https://www.dropbox.com/sh/ze928lqcusk64vs/AAD_qJcvuz6w5da8DiY7vuX1a?dl=0
That is the dropbox link to my Result images (some need to opened in ImageJ to work). There may be multipule images per sample so please pick the best ones. Any more questions let me know.

Thank you in advance :).