Mini Assignment # 4 This assignment is due February 10th, 2025 Make a figure with the following subpanels: Retrieve the Alphafold 2 model of Pseudechis australis basic phospholipase A2-PA5 from the Uniprot website for the protein sequence.

The model can be found lower down in the page, above the sequence itself. All of the following should be separate panels in a single figure, arranged as a 2×2 grid, assembled in Inkscape or a similar program.

Keep the size of the protein and orientation consistent between panels (you can use get_view/set_view, and save scenes as you go, as you might find that you need to use a slightly different view later). Use a white background, maximum detail, with no shadows, at high resolution (at least 1000 pixels per image).

Everything should be ray traced, and look crisp and professional, as in a paper. A) Using the MSA you developed for assignment #3, make a Consurf figure of the publicly available Alphafold 2 model of Pseudechis australis basic phospholipase A2-PA5, coloured by sequence conservation in Consurf. Do not use Consurf’s auto generated MSA, make sure to give it the one you have hand curated.

You can use any reasonable colour gradient for Consurf, as long as you describe it in the panel (Default cyan/magenta is fine too). It’s generally a good idea to keep neutrally conserved as white. Orient the molecule so that the most conserved surface faces the viewer, with the longest axis of the protein vertical. Label key active site residues to the extent that they are visible in the image (see below).

B) Perform a DALI search of the PDB using Pseudechis australis basic phospholipase A2. Superpose this structure on the top hit, using DALI for the superposition. Show a single chain of each as a cartoon, along with any bound ligands. Choose colours that contrast but don’t clash. Show only a single protomer shown for each protein. In the accompanying caption, note the Z-score, sequence identity, rmsd and aligned length. What is the oligomeric state of the protein you are superimposing on? C) Phospholipases use a membrane lipid as their substrate. Using OPM, predict the orientation of Pseudechis australis basic phospholipase A2 in the mammalian cellular membrane. Using information from papers from known homolog structures (part B), identify any key catalytic residues. Make a third figure, in the same orientation as the other two, showing the protein as a cartoon trace. Show disulfide bonds, active site residues, and any residues that extend into the plane of the membrane as sticks, side chain only. Colour each group of residues with the carbon atoms a distinct colour, and heteroatoms as their default colours. Show the cartoon of the protein in a fourth colour – white is a good default choice. Show the plane of the membrane as yellow transparent spheres. Make a second panel showing the protein in the plane of the membrane; this panel should be at 90 degrees to the first view – you can go back and adjust this view. Label the shown residues.

Provide an appropriate caption for each figure panel, explaining what you are showing, including which sequences were used to generate the Consurf figure. Make sure that AC are clearly marked in the figure.

Describe each of the required sections of the Leadership Development Plan. Please organize your Leadership Development Plan around the three sections with the section titles (e.g., “What is Leadership and What Kind of Leader Are You?”) serving as the main headings for your paper. Papers should be double spaced. You can refer to readings and videos, but do not need to include a references page.

Section 1: What is Leadership and What Kind of Leader Are You? (1-2 pages)

First, define leadership in your own words. Begin the paper by completing the sentence, “I define leadership as…”
What kind of leader are you today? (Be honest – this is your starting point)
What do you hope to achieve from the preparation of this document?
Section 2: Lessons Learned (3-5 pages)

What have you learned about yourself and others as a result of EACH of the four self-assessments you have completed? (One paragraph for each self-assessment)
What are your significant strengths as a leader of other people; that is, what are you exceptional at doing and/or helping others to do? Don’t be modest.

What do you need to improve? Don’t be easy on yourself.
Discuss how your strengths and developmental areas have helped and hindered you on a professional and/or personal basis.
Which 3 of the course articles or videos had a particular impact on you, and how will you change your behavior or decisions moving forward as a result?

If 20 or 30 years from now, people were to describe the kind of leader you were, what would you want that descriiption to include?
Section 3: Next Steps and Development Action Plan (1-2 pages; should include at least three specific, measurable action steps with target dates)

To continue your development as a leader, what are your action steps? EXAMPLE 1: What new habits can you implement? EXAMPLE 2: What experiences do you need to seek out? EXAMPLE 3: What types of relationships do you need to build? EXAMPLE 4: What training/education will you pursue (e.g., a specific LinkedIn Learning course). Get very specific in this section.
The Leadership Development Plan will be graded on the following criteria:

Integration of course concepts and theory. Demonstrate that you not only understand but can apply the conceptual ideas of the course in your plan.
Reflectiveness. Demonstrate a personal commitment to learn and change your ideas in light of experience and feedback from others.
Writing style. A paper will receive more weight if it is well-organized (edited) and well-written in terms of syntax and use of English. Please write this paper in first person, active voice.

 

As deputy director of the Sunnyside Health Department, part of your responsibility is education. Your agency has a project initiative that will require you and your employees to work collaboratively with individuals in different positions and different knowledge areas within the discipline of healthcare and public health. This collaboration is needed in order to create the appropriate public health intervention methods. You have been asked to write a white paper that communicates the importance of interprofessional collaboration.

INSTRUCTIONS

You are tasked with writing a white paper on the importance of interprofessional collaboration in public health. Discuss the core competencies, benefits, and challenges of implementing interprofessional collaboration practices for delivering the highest quality of healthcare for the public.
Define interprofessional collaboration and discuss its importance
Benefits
Challenges

Include suggestions for overcoming these challenges
Discuss interprofessional strategies and why they are important

Discuss how simulations aid in interprofessional collaboration
Benefits
Challenges

Discuss standardized tools

 

Please write interview based on resume and your own idea answering question. Galina is currently DNP, NP instructor and team leader in Stroke Neurology Unit in the hospital. She also own primary care, neurology vascular practice and practicing there too. Please create 5 -PPT Slides based on interview.

3. DNP Interview: Choose a DNP level leader to interview utilizing the following interview questions:

1. Descriiption of role in the organization.

2. Descriiption of preparation for the role – education, work history, previous roles, certification.

3. Descriiption of his/her competencies.

4. How does he/she compare with the Porter-O’Grady’s 10 principles of leaders in the Quantum Age (Chapter 2):
-view of systems as wholes not just the sum of parts
-health care is local; local relationships and not centralized authorities
-value as the centerpiece of service delivery
-simple systems aggregate to complex systems
-diversity is essential to life
-error is essential to success

-systems thrive when all their functions intersect and interact
-change is generated from the center outward
-revolution results from the aggregation of local changes

Select one option to complete the writing assignment
Option 1: Student Exercise 3.2 Determining if modern sport is a meritocracy (pg. 86)

Option2: Student Exercise 3.4 Proper role of physical education (pg. 91)
Requirements: Use the APA format, including a cover page, main body, and references. The main body should be at least three pages long, and at least three references are needed.